PUBLICATION
Drugs Affecting Phospholipase C-Mediated Signal Transduction Block the Olfactory Cyclic Nucleotide-Gated Current of Adult Zebrafish
- Authors
- Ma, L. and Michel, W.C.
- ID
- ZDB-PUB-980401-5
- Date
- 1998
- Source
- Journal of neurophysiology 79(3): 1183-1192 (Journal)
- Registered Authors
- Michel, William C.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cyclic AMP/pharmacology*
- Cysteine/pharmacology
- Evoked Potentials/drug effects
- Evoked Potentials/physiology
- Ion Channels/drug effects
- Ion Channels/physiology*
- Nasal Mucosa/drug effects
- Nasal Mucosa/innervation
- Nasal Mucosa/physiology
- Neomycin/pharmacology
- Odorants*
- Olfactory Pathways/drug effects
- Olfactory Pathways/physiology*
- Olfactory Receptor Neurons/drug effects
- Olfactory Receptor Neurons/physiology*
- Patch-Clamp Techniques
- Ruthenium Red/pharmacology
- Signal Transduction/drug effects*
- Signal Transduction/physiology
- Taurocholic Acid/pharmacology
- Type C Phospholipases/metabolism*
- Zebrafish
- PubMed
- 9497400 Full text @ J. Neurophysiol.
Citation
Ma, L. and Michel, W.C. (1998) Drugs Affecting Phospholipase C-Mediated Signal Transduction Block the Olfactory Cyclic Nucleotide-Gated Current of Adult Zebrafish. Journal of neurophysiology. 79(3):1183-1192.
Abstract
Amino acid and bile salt odorants are detected by zebrafish with relatively independent odorant receptors, but the transduction cascade(s) subsequently activated by these odorants remains unknown. Electro-olfactogram recording methods were used to determine the effects of two drugs, reported to affect phospholipase C (PLC)/inositol tripohsphate (IP3)-mediated olfactory transduction in other vertebrate species, on amino acid and bile salt-evoked responses. At the appropriate concentrations, either an IP3-gated channel blocker, ruthenium red (0.01-0.1 microM), or a PLC inhibitor, neomycin (50 microM), reduced amino-acid-evoked responses to a significantly greater extent than bile salt-evoked responses. Excised patch recording techniques were used to measure the affects of these drugs on second-messenger-activated currents. Ruthenium red and neomycin are both effective blockers of the olfactory cyclic nucleotide-gated (CNG) current. Both drugs blocked the CNG channel in a voltage-dependent and reversible manner. No IP3-activated currents could be recorded. The differential effects of ruthenium red and neomycin on odor-evoked responses suggest the activation of multiple transduction cascades. The nonspecific actions of these drugs on odor-activated transduction pathways and our inability to record an IP3-activated current do not permit the conclusion that zebrafish, like other fish species, use a PLC/IP3-mediated transduction cascade in the detection of odorants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping