PUBLICATION

Segmental expression of Hoxb-1 is controlled by a highly conserved autoregulatory loop dependent upon exd/pbx

Authors
Pöpperl, H., Bienz, M., Studer, M., Chan, S., Aparicio, S., Brenner, S., Mann, R., and Krumlauf, R.
ID
ZDB-PUB-980317-4
Date
1995
Source
Cell   81: 1031-1042 (Journal)
Registered Authors
Aparicio, Sam, Chan, Shu Jin, Krumlauf, Robb
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Chickens
  • Consensus Sequence
  • Conserved Sequence
  • Drosophila/embryology
  • Drosophila/metabolism
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/physiology
  • Embryo, Nonmammalian/metabolism
  • Enhancer Elements, Genetic
  • Fishes
  • Gene Expression*
  • Genes, Homeobox*
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Multigene Family*
  • Recombinant Proteins/biosynthesis
  • Regulatory Sequences, Nucleic Acid*
  • Restriction Mapping
  • Rhombencephalon/metabolism*
  • Sequence Homology, Nucleic Acid
  • Vertebrates
PubMed
7600572 Full text @ Cell
Abstract
Comparison of Hoxb-1 regulatory regions from different vertebrates identified three related sequence motifs critical for rhombomere 4 (r4) expression in the hindbrain. Functional analysis in transgenic mice and Drosophila embryos demonstrated that the conserved elements are involved in a positive autoregulatory loop dependent on labial (lab) family members. Binding of Hoxb-1 to these elements in vitro requires cofactors, and the motifs closely resemble the consensus binding site for pbx1, a homolog of the Drosophila extradenticle (exd) homoedomain protein. In vitro exd/pbx serves as a Hoxb-1 cofactor in cooperative binding and in Drosophila expression mediated by the r4 enhancer is dependent on both lab and exd. This provides in vivo and in vitro evidence that r4 expression involves direct autoregulation dependent on cooperative interactions of Hoxb-1 with exd/pbx proteins as cofactors.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping