PUBLICATION

Introducing foreign genes into fish eggs with electroporated sperm as a carrier

Authors
Müller, F., Ivics, Z., Erdelyi, F. Papp, T., Varadi, L., Horvath, L., Maclean, N., and Orbán, L.
ID
ZDB-PUB-980224-4
Date
1992
Source
Molecular marine biology and biotechnology   1: 276-281 (Journal)
Registered Authors
Erdelyi, Ferenc, Horvath, Laszlo, Ivics, Zoltan, Maclean, Norman, Müller, Ferenc, Orban, Laszlo, Váradi, László
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified/genetics*
  • Carps/genetics
  • Catfishes/genetics
  • Cytological Techniques
  • DNA/analysis
  • Female
  • Fertilization
  • Fishes/genetics*
  • Gene Expression
  • Genetic Vectors
  • Male
  • Ovum/physiology
  • Sperm Motility
  • Spermatozoa/physiology*
  • Tilapia/genetics
  • Transfection/methods*
PubMed
1308817
Abstract
A new method has been developed for introduction of foreign genes into fish eggs. The procedure is based on the incubation of fish sperm cells suspended in dilute citrate solution with plasmid DNA, followed by application of high-field-strength electrical pulses (electroporation) to increase DNA binding., uptake, or both. Tissue homogenates and genomic DNA extracts of free swimming fry developed from eggs fertilized with treated sperm was tested to evaluate the efficiency of gene transfer. Dot blot hybridization and gene expression assay demonstrated the presence and expression of the reporter genes introduced in 2.6 to 4.2% of several hundreds of tested larvae of common carp (Cyprinus carpio L.), African catfish (Clarias gariepinus), and tilapia (Oreochromis niloticus). No transgene has been found in the fry resulting from parallel experiments without sperm electroporation. This is the first report on successful application of electroporated sperm cells for production of transgenic fish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes