ZFIN ID: ZDB-PUB-980209-1
Positional cloning identifies zebrafish one-eyed pinhead as a permissive EGF-related ligand required during gastrulation
Zhang, J., Talbot, W.S., and Schier, A.F.
Date: 1998
Source: Cell   92: 241-251 (Journal)
Registered Authors: Schier, Alexander, Talbot, William S., Zhang, Jiaojiao
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Cell Membrane/chemistry
  • Central Nervous System/embryology
  • Cloning, Molecular/methods*
  • Endoderm
  • Epidermal Growth Factor/genetics
  • Gastrula/chemistry
  • Gastrula/physiology*
  • Gene Expression Regulation, Developmental
  • Genes/genetics
  • Homeodomain Proteins/analysis
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/physiology
  • Ligands
  • Molecular Sequence Data
  • Mutation
  • Protein Sorting Signals/genetics
  • RNA, Messenger/analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors/analysis
  • Transcription Factors/genetics*
  • Transcription Factors/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 9458048 Full text @ Cell
The zebrafish one-eyed pinhead (oep) mutation disrupts embryonic development, resulting in cyclopia and defects in endoderm, prechordal plate, and ventral neuroectoderm formation. We report the molecular isolation of oep using a positional cloning approach. The oep gene encodes a novel EGF- related protein with similarity to the EGF-CFC proteins cripto, cryptic, and FRL-1. Wild-type oep protein contains a functional signal sequence and is membrane-associated. Following ubiquitous maternal and zygotic expression, highest levels of oep mRNA are found in the gastrula margin and in axial structures and forebrain. Widespread misexpression of both membrane-attached and secreted forms of oep rescues prechordal plate and forebrain development in mutant embryos but does not lead to the ectopic induction of these cell types in wild-type fish. These results establish an essential but permissive role for an EGF-related ligand during vertebrate gastrulation.