ZFIN ID: ZDB-PUB-971203-8
A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula
Fürthauer, M., Thisse, C., and Thisse, B.
Date: 1997
Source: Development (Cambridge, England)   124(21): 4253-4264 (Journal)
Registered Authors: Fürthauer, Maximilian, Thisse, Bernard, Thisse, Christine
Keywords: fgf-8; Spemann's organizer; gastrulation; dorsoventral patterning; convergence; BMP2; BMP4; Chordin; Noggin; Goosecoid
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Blastocyst/physiology
  • Body Patterning/genetics*
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Cloning, Molecular
  • Embryo, Nonmammalian
  • Embryonic Induction/genetics
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors*
  • Gastrula/physiology*
  • Gene Expression Regulation, Developmental
  • Growth Substances/physiology*
  • Mesencephalon/embryology
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Transforming Growth Factor beta*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins
PubMed: 9334274
Signals released from Spemann's organizer, together with ventralizing factors such as BMPs, are necessary to pattern the dorsoventral axis of the vertebrate embryo. We report that a member of the FGF family, fgf-8, not secreted by the axial mesoderm but expressed in a dorsoventral gradient at the margin of the zebrafish gastrula, also contributes to the establishment of the dorsoventral axis of the embryo. Ectopic expression of FGF-8 leads to the expansion of dorsolateral derivatives at the expense of ventral and posterior domains. Moreover, FGF-8 displays some organizer properties as it induces the formation of a partial secondary axis in the absence of factors released from Spemann's organizer territory. Analysis of its interaction with the ventralizing factors, BMPs, reveals that overexpression of FGF-8 inhibits the expression of these factors in the ventral part of the embryo as early as blastula stage, suggesting that FGF-8 acts upstream of BMP2 and BMP4. We conclude that FGF-8 is involved in defining dorsoventral identity and is an important organizing factor responsible for specification of mesodermal and ectodermal dorsolateral territories of the zebrafish gastrula.