PUBLICATION
Vsx-1 and Vsx-2: differential expression of two paired-like homeobox genes during zebrafish and goldfish retinogenesis
- Authors
- Passini, M.A., Levine, E.M., Canger, A.K., Raymond, P.A., and Schechter, N.
- ID
- ZDB-PUB-971203-5
- Date
- 1997
- Source
- The Journal of comparative neurology 388(3): 495-505 (Journal)
- Registered Authors
- Canger, Anthony K., Levine, Elena, Passini, Marco, Raymond, Pamela, Schechter, Nisson
- Keywords
- eye; retina; ocular retardation; CVC domain; development
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Eye/embryology
- Eye Proteins/genetics*
- Fish Proteins*
- Gene Expression Regulation, Developmental/physiology*
- Genes, Homeobox*
- Goldfish/embryology
- Goldfish/genetics*
- Homeodomain Proteins/genetics*
- Molecular Sequence Data
- Retina/embryology
- Retina/metabolism*
- Sequence Homology, Amino Acid
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 9368856 Full text @ J. Comp. Neurol.
Citation
Passini, M.A., Levine, E.M., Canger, A.K., Raymond, P.A., and Schechter, N. (1997) Vsx-1 and Vsx-2: differential expression of two paired-like homeobox genes during zebrafish and goldfish retinogenesis. The Journal of comparative neurology. 388(3):495-505.
Abstract
Vsx-1 and Vsx-2 are two homeobox genes that were cloned originally from an adult goldfish retinal library. They are members of the paired-like:CVC gene family, which is characterized by the presence of a paired homeodomain and an additional conserved region, termed the CVC domain. To analyze the possible roles for Vsx-1 and Vsx-2 in eye development, we used in situ hybridization to examine their expression patterns in zebrafish and goldfish embryos. Vsx-2 is initially expressed by proliferating neuroepithelial cells of the presumptive neural retina, then it is down-regulated as differentiation begins, and it is finally reexpressed at later stages of differentiation in a subset of cells, presumed to be bipolar cells, in the inner nuclear layer. In contrast, Vsx-1 is expressed only weakly in undifferentiated, presumptive neural retina and is then up-regulated selectively in presumptive bipolar cells at early stages of differentiation (when Vsx-2 is turned off), before decreasing to an intermediate level, which is maintained in the differentiated (adult) retina. The restricted expression patterns of Vsx-2 correspond to the observed phenotypes in mice with the ocular retardation mutation (orJ), further supporting the notion that Vsx-2 and Chx10 are homologues. The sequential complimentary and then corresponding expression patterns of Vsx-1 and Vsx-2 suggest that these similar transcription factors may be recruited for partially overlapping, but distinct, functions during the development of the retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping