PUBLICATION
A vasa-like gene in zebrafish identifies putative primordial germ cells
- Authors
- Olsen, L.C., R. Aasland, and A. Fjose
- ID
- ZDB-PUB-971103-6
- Date
- 1997
- Source
- Mechanisms of Development 66(1-2): 95-105 (Journal)
- Registered Authors
- Aasland, Rein, Fjose, Anders
- Keywords
- primordial germ cells; vasa; vasa-like gene; RNA helicase; zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- DEAD-box RNA Helicases/chemistry
- DEAD-box RNA Helicases/genetics*
- DEAD-box RNA Helicases/isolation & purification
- DNA, Complementary/isolation & purification
- Gene Expression Regulation, Developmental*
- Germ Cells/chemistry
- Germ Cells/metabolism*
- Molecular Sequence Data
- RNA Helicases*
- RNA Nucleotidyltransferases/chemistry
- RNA Nucleotidyltransferases/genetics*
- RNA Nucleotidyltransferases/isolation & purification
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/isolation & purification
- PubMed
- 9376327 Full text @ Mech. Dev.
Citation
Olsen, L.C., R. Aasland, and A. Fjose (1997) A vasa-like gene in zebrafish identifies putative primordial germ cells. Mechanisms of Development. 66(1-2):95-105.
Abstract
The vasa gene is essential for germline formation in Drosophila. Vasa-related genes have been isolated from several organisms including nematode, frog and mammals. In order to gain insight into the early events in vertebrate germline development, zebrafish was chosen as a model. Two zebrafish vasa-related genes were isolated, pl10a and vlg. The pl10a gene was shown to be widely expressed during embryogenesis. The vlg gene and vasa belong to the same subfamily of RNA helicase encoding genes. Putative maternal vlg transcripts were detected shortly after fertilization and from the blastula stage onwards, expression was restricted to migratory cells most likely to be primordial germ cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping