PUBLICATION
Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog
- Authors
- Blagden, C.S., P.D. Currie, P.W. Ingham, and S.M. Hughes
- ID
- ZDB-PUB-971103-2
- Date
- 1997
- Source
- Genes & Development 11(17): 2163-2175 (Journal)
- Registered Authors
- Blagden, Chris, Currie, Peter D., Hughes, Simon M., Ingham, Philip
- Keywords
- zebrafish; muscle; fiber type; adaxial cells; sonic hedgehog; myoblast
- MeSH Terms
-
- Animals
- Biological Evolution
- Embryonic Induction/genetics
- Embryonic Induction/physiology*
- Hedgehog Proteins
- Immunohistochemistry
- Mesoderm/cytology
- Muscle Fibers, Fast-Twitch/cytology
- Muscle Fibers, Fast-Twitch/metabolism
- Muscle Fibers, Slow-Twitch/cytology*
- Muscle Fibers, Slow-Twitch/metabolism
- Mutation
- Myosin Heavy Chains/metabolism
- Notochord/embryology*
- Proteins/genetics
- Proteins/physiology*
- Time Factors
- Trans-Activators*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 9303533 Full text @ Genes & Dev.
Citation
Blagden, C.S., P.D. Currie, P.W. Ingham, and S.M. Hughes (1997) Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog. Genes & Development. 11(17):2163-2175.
Abstract
The patterning of vertebrate somitic muscle is regulated by signals from neighboring tissues. We examined the generation of slow and fast muscle in zebrafish embryos and show that Sonic hedgehog (Shh) secreted from the notochord can induce slow muscle from medial cells of the somite. Slow muscle derives from medial adaxial myoblasts that differentiate early, whereas fast muscle arises later from a separate myoblast pool. Mutant fish lacking shh expression fail to form slow muscle but do form fast muscle. Ectopic expression of shh, either in wild-type or mutant embryos, leads to ectopic slow muscle at the expense of fast. We suggest that Shh acts to induce myoblasts committed to slow muscle differentiation from uncommitted presomitic mesoderm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping