PUBLICATION
Efficient detection of DNA polymorphisms by fluorescent RAPD analysis
- Authors
- Corley-Smith, G.E., Lim, C.J., Kalmar, G.B., and Brandhorst, B.P.
- ID
- ZDB-PUB-970918-13
- Date
- 1997
- Source
- Biotechniques 22(4): 690-692 (Journal)
- Registered Authors
- Brandhorst, Bruce P., Corley-Smith, Graham, Lim, Chinten James
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA/analysis
- DNA/genetics*
- DNA Primers
- Electrophoresis, Agar Gel
- Electrophoresis, Polyacrylamide Gel
- Ethidium
- Fluorescence
- Genetic Markers/genetics
- Polymerase Chain Reaction
- Polymorphism, Genetic*
- Random Amplified Polymorphic DNA Technique*
- Zebrafish
- PubMed
- 9105621 Full text @ Biotechniques
Citation
Corley-Smith, G.E., Lim, C.J., Kalmar, G.B., and Brandhorst, B.P. (1997) Efficient detection of DNA polymorphisms by fluorescent RAPD analysis. Biotechniques. 22(4):690-692.
Abstract
A method is presented for the analysis of fluorescently labeled random amplified polymorphic DNA (FRAPD) fragments. A DNA sequencer and collection and analysis software were used to estimate the sizes of DNA fragments based on their mobilities relative to in-lane size markers. This allowed confident identification and comparison of FRAPD markers both within and between polyacrylamide gels. In comparison with analysis of RAPD products using ethidium bromide-stained agarose gels, fluorescent analysis improved the sensitivity, resolution and precision of sizing of RAPD products of about 50-2100 bp. FRAPD fragments produced from amplification of zebrafish DNA are informative as genetic markers that segregate with Mendelian inheritance. FRAPD analysis was found to be very efficient for identifying new DNA polymorphisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping