PUBLICATION
Novel Eph-family receptor tyrosine kinase is widely expressed in the developing zebrafish nervous system
- Authors
- Bovenkamp, D.E. and Greer, P.
- ID
- ZDB-PUB-970618-71
- Date
- 1997
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 209(2): 166-181 (Journal)
- Registered Authors
- Bovenkamp, Diane, Greer, Peter A.
- Keywords
- receptor tyrosine kinase; Eph; zebrafish; nervous system; patterning; development
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Central Nervous System/embryology
- Central Nervous System/enzymology*
- Cloning, Molecular
- DNA
- Gene Expression Regulation, Developmental
- Molecular Sequence Data
- Receptor Protein-Tyrosine Kinases/genetics*
- Receptors, Eph Family*
- Transcription, Genetic
- Transfection
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 9186052 Full text @ Dev. Dyn.
Citation
Bovenkamp, D.E. and Greer, P. (1997) Novel Eph-family receptor tyrosine kinase is widely expressed in the developing zebrafish nervous system. Developmental Dynamics : an official publication of the American Association of Anatomists. 209(2):166-181.
Abstract
In a search for novel tyrosine kinases involved in vertebrate development, we have isolated cDNAs corresponding to three distinct members of the Eph-family of receptor tyrosine kinases. Whole mount RNA in situ hybridization analysis showed all three genes were most abundantly expressed in the developing nervous system. zek1 (zebrafish Eph-like kinase1) encodes a 981 amino acid polypeptide closely related to the murine Sek1 and Bsk receptors. Cos-1 cells transfected with zek1 produce a 141 kilodalton tyrosine phosphorylated protein which is recognized by antibodies raised against two predicted Zek1 peptides. These antibodies also recognized a protein of the same apparent molecular weight in lysates from zebrafish embryos and adults. Widespread expression of zek1 in the developing brain and neural tube suggested a generalized function of the Zek1 receptor in neuronal cell ontogeny.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping