PUBLICATION
Loss of cerebum function ventralizes the zebrafish embryo
- Authors
- Fisher, S., Amacher, S.L., and Halpern, M.E.
- ID
- ZDB-PUB-970602-1
- Date
- 1997
- Source
- Development (Cambridge, England) 124(7): 1301-1311 (Journal)
- Registered Authors
- Amacher, Sharon, Fisher, Shannon, Halpern, Marnie E.
- Keywords
- gastrulation; mesoderm; organizer; cerebum; zebrafish
- MeSH Terms
-
- Alleles
- Animals
- Body Patterning/genetics*
- Central Nervous System/embryology
- Chromosome Mapping
- Ectoderm/physiology
- Embryonic Induction/genetics*
- Gastrula/physiology
- Mesoderm/physiology
- Mutation*
- Phenotype
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 9118801 Full text @ Development
Citation
Fisher, S., Amacher, S.L., and Halpern, M.E. (1997) Loss of cerebum function ventralizes the zebrafish embryo. Development (Cambridge, England). 124(7):1301-1311.
Abstract
Recent studies implicate ventrally derived signals, in addition to dorsal ones emanating from the organizer, in patterning the vertebrate gastrula. We have identified five overlapping deficiencies that uncover the zebrafish cerebum locus and dramatically alter dorsal-ventral polarity at gastrulation. Consistent with the properties of experimentally ventralized amphibian embryos, cerebum mutants exhibit reduced neurectodermal gene expression domains and an increase in derivatives of ventral mesoderm. Structures derived from paraxial and lateral mesoderm also are reduced; however, dorsal axial mesodermal derivatives, such as the hatching gland and notochord, are largely spared. The pleiotropic action of cerebum deficiencies, and the differential response of affected tissues, suggest that the cerebum gene may normally function as an inhibitor of ventralizing signals, a function previously ascribed to Noggin and Chordin in Xenopus. Analysis of the cerebum phenotype provides genetic evidence for the existence of ventralizing signals in the zebrafish gastrula and for antagonists of those signals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping