PUBLICATION
Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos
- Authors
- Conway, G., Margoliath, A., Wong-Madden, S., Roberts, R.J., and Gilbert, W.
- ID
- ZDB-PUB-970429-63
- Date
- 1997
- Source
- Proceedings of the National Academy of Sciences of the United States of America 94(7): 3082-3087 (Journal)
- Registered Authors
- Conway, Greg, Gilbert, Walter
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement*
- Cloning, Molecular
- DNA-Binding Proteins/genetics
- Embryo, Nonmammalian/cytology*
- Gene Expression Regulation, Developmental
- Gene Expression Regulation, Enzymologic
- Goosecoid Protein
- Homeodomain Proteins*
- Janus Kinase 1
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Protein-Tyrosine Kinases/genetics
- Protein-Tyrosine Kinases/metabolism*
- Repressor Proteins*
- Transcription Factors*
- Zebrafish/embryology*
- Zebrafish Proteins
- PubMed
- 9096349 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Conway, G., Margoliath, A., Wong-Madden, S., Roberts, R.J., and Gilbert, W. (1997) Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos. Proceedings of the National Academy of Sciences of the United States of America. 94(7):3082-3087.
Abstract
Establishment of the vertebrate body plan requires a variety of signaling molecules. In a search for tyrosine kinases expressed in early zebrafish embryos, a model system for the study of vertebrate development, we discovered Jak1 kinase to be maternally encoded and the mRNA evenly distributed among the cells of blastula-stage embryos. Injection of RNA-encoding dominant-negative Jak1 kinases reduces a specific cell migration, epiboly, and results in the reduction of goosecoid expression and of anterior structures. This work establishes that, in addition to its role in signal transduction of cytokines in adult tissues, Jak1 kinase has a role in early vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping