PUBLICATION
A complement factor B-like cDNA clone from the zebrafish (Brachydanio rerio)
- Authors
- Seeger, A., Mayer, W.E., and Klein, J.
- ID
- ZDB-PUB-970326-9
- Date
- 1996
- Source
- Molecular immunology 33(6): 511-520 (Journal)
- Registered Authors
- Klein, Jan, Mayer, Werner E.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Complement C2/genetics
- Complement Factor B/genetics*
- DNA Primers/chemistry
- DNA, Complementary/genetics
- Gene Library
- Humans
- Molecular Sequence Data
- Phylogeny
- Sequence Alignment
- Sequence Homology, Amino Acid
- Zebrafish/genetics*
- PubMed
- 8700167 Full text @ Mol. Immunol.
Citation
Seeger, A., Mayer, W.E., and Klein, J. (1996) A complement factor B-like cDNA clone from the zebrafish (Brachydanio rerio). Molecular immunology. 33(6):511-520.
Abstract
An important molecule in the activation of the complement system in vertebrates is factor B, a serine protease with a molecular mass of 95,000. Factor B and the complement component C2 are thought to have arisen by gene duplication. In mammals and in Xenopus the factor B gene is linked to the major histocompatibility complex (MHC), whereas in domestic fowl it segregates independently of the MHC. Here we describe the isolation of a cDNA clone coding for factor B in the zebrafish, Brachydanio rerio. The deduced protein sequence exhibits a characteristic mosaic structure consisting of the short consensus repeat (SCR), the von Willebrand factor, and the serine protease domains. The estimated time of factor B and C2 divergence (approximately 350 million years ago), combined with the fact that C2 has thus far been found only in mammals, suggest that the factor B-C2 gene duplication occurred after the divergence of mammal-like reptiles from other reptiles and hence also birds. After the duplication, the C2 component evolved significantly faster than factor B.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping