PUBLICATION
Expression of a Na,K-ATPase Beta3 subunit during development of the zebrafish central nervous system
- Authors
- Appel, C., Gloor, S., Schmalzing, G., Schachner, M., and Bernhardt, R.R.
- ID
- ZDB-PUB-970103-1
- Date
- 1996
- Source
- Journal of neuroscience research 46(5): 551-564 (Journal)
- Registered Authors
- Bernhardt, Robert, Schachner, Melitta
- Keywords
- adhesion molecule on glia (AMOG); sodium pump; cell recognition; embryogenesis; differentiation
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Differentiation
- DNA, Complementary/genetics
- Genes
- In Situ Hybridization
- Larva
- Molecular Sequence Data
- Nerve Tissue Proteins/biosynthesis
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/genetics*
- Oocytes
- RNA, Messenger/biosynthesis
- Sequence Alignment
- Sodium-Potassium-Exchanging ATPase/biosynthesis
- Sodium-Potassium-Exchanging ATPase/chemistry
- Sodium-Potassium-Exchanging ATPase/genetics*
- Xenopus laevis
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 8951667 Full text @ J. Neurosci. Res.
Citation
Appel, C., Gloor, S., Schmalzing, G., Schachner, M., and Bernhardt, R.R. (1996) Expression of a Na,K-ATPase Beta3 subunit during development of the zebrafish central nervous system. Journal of neuroscience research. 46(5):551-564.
Abstract
Zebrafish beta 3, a full length cDNA clone encoding a zebrafish Na,K-ATPase beta subunit, was isolated. The protein shares highest homology with the beta 3 subunits of amphibians and mammals, slightly less homology with the beta 2 subunits, and is distinct from the beta 1 subunits. The fish beta subunit co-assembled with alpha subunits to form Na,K-ATPase enzymes when expressed in Xenopus oocytes. Embryonic expression was first detected by whole-mount in situ hybridization between 8-12 hr post-fertilization (hpf) in the head mesoderm. Subsequently, and up to 24 hpf, the mRNA was confined to four dorsal domains in the anterior neural tube. After a transient downregulation during the second day, expression was again conspicuous in the nervous system of 3-day-old larvae. Based on its distribution pattern, the fish beta subunit could be involved in setting up regional identities in the developing fish CNS and in the differentiation of distinct cell types
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping