PUBLICATION
Fluorescence-based signal amplification technology
- Authors
- Singer, V.L., Paragas, V.B., Larison, K.D., Wells, K.S., Fox, C.J., and Haugland, R.P.
- ID
- ZDB-PUB-970102-8
- Date
- 1994
- Source
- American biotechnology laboratory 12(11): 55-6 (Journal)
- Registered Authors
- Haugland, Richard P., Larison, Karen D., Singer, V.L.
- Keywords
- none
- MeSH Terms
-
- Alkaline Phosphatase/chemistry*
- Animals
- Concanavalin A/metabolism
- Fibroblasts/metabolism
- Flow Cytometry
- Fluorescent Dyes/chemistry*
- Humans
- Immunoblotting
- Immunohistochemistry
- In Situ Hybridization
- Mice
- RNA, Messenger/analysis
- Spectrometry, Fluorescence*
- Tumor Cells, Cultured
- Zebrafish
- PubMed
- 7765427
Citation
Singer, V.L., Paragas, V.B., Larison, K.D., Wells, K.S., Fox, C.J., and Haugland, R.P. (1994) Fluorescence-based signal amplification technology. American biotechnology laboratory. 12(11):55-6.
Abstract
The ELF alkaline phosphate substrate can be used to fluorescently label a wide variety of biological targets. This substrate yields a bright, photostable yellow-green fluorescent precipitate at the site of enzymatic activity. ELF labeling can be as much as 40 times as bright and hundreds of times as photostable as labeling with conventional fluorophores and yields signals capable of very fine submicroscopic resolution. Signal development is also extremely rapid, making the signal amplification technology well suited for applications such as RNA in situ hybridization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping