ZFIN ID: ZDB-PUB-961220-1
Retinoic acid alters photoreceptor development in vivo
Hyatt, G.A., Schmitt, E.A., Fadool, J.M., and Dowling, J.E.
Date: 1996
Source: Proceedings of the National Academy of Sciences of the United States of America   93(23): 13298-13303 (Journal)
Registered Authors: Dowling, John E., Fadool, James M., Hyatt, George, Schmitt, Ellen
Keywords: retina; rod photoreceptor; cone photoreceptor; zebrafish; teleost
MeSH Terms:
  • Animals
  • Cell Division/drug effects
  • Embryo, Nonmammalian
  • Immunohistochemistry
  • Photoreceptor Cells/drug effects
  • Photoreceptor Cells/embryology*
  • Reference Values
  • Retinal Cone Photoreceptor Cells/cytology
  • Retinal Cone Photoreceptor Cells/drug effects
  • Retinal Cone Photoreceptor Cells/embryology
  • Retinal Rod Photoreceptor Cells/cytology
  • Retinal Rod Photoreceptor Cells/drug effects
  • Retinal Rod Photoreceptor Cells/embryology
  • Rod Opsins/biosynthesis*
  • Transcription, Genetic/drug effects
  • Tretinoin/pharmacology*
  • Zebrafish
PubMed: 8917585 Full text @ Proc. Natl. Acad. Sci. USA
Application of exogenous retinoic acid (RA) to zebrafish during the initial stages of photoreceptor differentiation results in a precocious development of rod photoreceptors and an inhibition of cone photoreceptor maturation. The acceleration of rod differentiation is observed initially within the ventral retina 3 days after fertilization, following 24 hr of RA application, and within the dorsal retina 4 days after fertilization, following 48 hr of RA application. The differentiation of rods was impeded significantly when the synthesis of endogenous retinoic acid was inhibited by citral prior to the initial stage of rod differentiation. RA-treated embryos labeled for bromodeoxyuridine (BrdU) uptake revealed that RA exerts its effect on a postmitotic cell population within the developing retina. During normal development in zebrafish, rod differentiation is most robust within the ventral retina, a region previously shown to be rich in RA. Our data suggest that the RA signaling pathway is involved in the differentiation and maturation of both the rod and cone photoreceptors within the developing zebrafish retina.