ZFIN ID: ZDB-PUB-961101-36
Derivation and characterization of a zebrafish liver cell line
Ghosh, C., Zhou, Y.L., and Collodi, P.
Date: 1994
Source: Cell biology and toxicology   10: 167-176 (Journal)
Registered Authors: Collodi, Paul, Ghosh, Chandramallika
Keywords: none
MeSH Terms:
  • Albumins/biosynthesis
  • Alkaline Phosphatase/biosynthesis
  • Animals
  • Aspartate Aminotransferases/biosynthesis
  • Cell Division
  • Cell Line*
  • Culture Media
  • Glucose-6-Phosphatase/biosynthesis
  • Karyotyping
  • Liver/cytology*
  • Liver/metabolism
  • Zebrafish*
PubMed: 7994634 Full text @ Cell Biol. Toxicol.
ABSTRACT
ZF-L cells were derived from normal adult zebrafish liver, and have been growing in culture for more than 100 generations. The cells were derived in basal nutrient medium supplemented with fetal bovine serum (FBS), trout serum, trout embryo extract, bovine insulin and mouse epidermal growth factor. After 50 generations in culture, optimal growth of the cells was achieved in medium supplemented with FBS (5%) and trout serum (0.5%). ZF-L cells were hypodiploid (modal chromosome number = 46) and exhibited an epithelial morphology. ZF-L cell homogenates exhibited alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. The cells synthesized and released several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG.
ADDITIONAL INFORMATION No data available