PUBLICATION

Developmental expression of class III and IV POU domain genes in the zebrafish

Authors
Sampath, K. and Stuart, G.W.
ID
ZDB-PUB-961014-971
Date
1996
Source
Biochemical and Biophysical Research Communications   219: 565-571 (Journal)
Registered Authors
Sampath, Karuna, Stuart, Gary
Keywords
none
MeSH Terms
  • Brain/embryology
  • Brain/metabolism*
  • Mammals
  • DNA Primers
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics*
  • Embryo, Nonmammalian/physiology
  • Base Sequence
  • Eye/embryology
  • Eye/metabolism
  • Gene Expression Regulation, Developmental*
  • Genes, Homeobox
  • Zebrafish
  • Animals
  • Conserved Sequence
  • Cloning, Molecular
  • Recombinant Proteins/biosynthesis
  • Recombinant Proteins/isolation & purification
  • Amino Acid Sequence
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
PubMed
8605028 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Using redundant primers in a PCR based cloning strategy, POU- domain encoding sequences representing two different class III genes (brain-1.1, brain-1.2) and one class IV gene (brain-3.1) were identified in zebrafish. Using the PCR clones to screen a zebrafish gastrula cDNA library, a third class III POU gene was identified (brain-1.0). RT-PCR assays performed with gene-specific primers indicated that the four POU genes exhibited distinct patterns of expression in developing embryos and adult fish. Like their mammalian homologs, the class III genes were expressed early in development and in the adult brain, while the class IV gene was poorly expressed during early development but highly expressed in the adult eye. Since two of the POU PCR clones were obtained from genomic DNA, the fish genes may also be similar to their mammalian homologs in being largely devoid of introns.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping