PUBLICATION
Expression of rod and cone visual pigments in goldfish and zebrafish: a rhodopsin-like gene is expressed in cones
- Authors
- Raymond, P.A., Barthel, L.K., Rounsifer, M.E., Sullivan, S.A., and Knight, J.K.
- ID
- ZDB-PUB-961014-923
- Date
- 1993
- Source
- Neuron 10: 1161-1174 (Journal)
- Registered Authors
- Barthel, Linda, Raymond, Pamela
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Expression*
- Goldfish/metabolism*
- In Situ Hybridization
- Light
- Photoreceptor Cells/metabolism*
- RNA Probes
- Retina/cytology
- Retina/physiology
- Retinal Pigments/biosynthesis*
- Retinal Pigments/genetics
- Rhodopsin/biosynthesis*
- Rhodopsin/genetics*
- Zebrafish/metabolism*
- PubMed
- 8318234 Full text @ Neuron
Citation
Raymond, P.A., Barthel, L.K., Rounsifer, M.E., Sullivan, S.A., and Knight, J.K. (1993) Expression of rod and cone visual pigments in goldfish and zebrafish: a rhodopsin-like gene is expressed in cones. Neuron. 10:1161-1174.
Abstract
The primary purpose of the present study was to determine whether a rhodopsin-like gene, which has been postulated to represent the green cone pigment in several species, is in fact expressed in cone photoreceptors instead of rods. The expression patterns of rod opsin and blue and red cone opsins were also examined in both goldfish and zebrafish retinas using colorimetric in situ hybridization. The results demonstrate that the rhodopsin-like gene is expressed in green cones, as predicted. A subset of small cones that do not hybridize with these cRNA probes are tentatively identified as ultraviolet receptors. The results also demonstrate that opsin message in cones is restricted to the perinuclear region, whereas in rods, it is both perinuclear and adjacent to the ellipsoid.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping