PUBLICATION
Developmental patterning of rod and cone photoreceptors in embryonic zebrafish
- Authors
- Raymond, P.A., Barthel, L.K., and Curran, G.A.
- ID
- ZDB-PUB-961014-922
- Date
- 1995
- Source
- The Journal of comparative neurology 359: 537-550 (Journal)
- Registered Authors
- Barthel, Linda, Raymond, Pamela
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Immunohistochemistry
- In Situ Hybridization
- Photoreceptor Cells/embryology*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 7499546 Full text @ J. Comp. Neurol.
Citation
Raymond, P.A., Barthel, L.K., and Curran, G.A. (1995) Developmental patterning of rod and cone photoreceptors in embryonic zebrafish. The Journal of comparative neurology. 359:537-550.
Abstract
Cone photoreceptors in the zebrafish retina are arranged in a crystalline lattice, with each spectral subtype at a specific position in the array; rod photoreceptors are inserted around the cones. Patterning events and developmental mechanisms that lead to the formation of the cone mosaic are not known. To begin investigating this issue, we examined the initial stages of opsin expression in zebrafish embryos by in situ hybridization with goldfish opsin cRNA probes to determine how and when the cone mosaic pattern arises. We found both differences and similarities in the spatiotemporal patterns of rod and cone development, which suggest the following: 1) Expression of opsin message (including rod opsin, blue and red cone opsins) was found in a ventral patch of retina located nasal to the choroid fissure. 2) The cone mosaic pattern was generated by a crystallization-like process initiated in the precocial ventral patch and secondarily in nasal retina, which then swept like a wave into dorsotemporal retina. 3) The remainder of the retina, suggesting that these precocial rods might differ from typical rods. 4) Developmental maturation of rods in zebrafish, as reflected by expression of opsin, may be accelerated compared to cones, which are thought to become postmitotic before rods. These data are consistent with a model in which lateral inductive interactions among differentiating photoreceptors lead to patterning of the array.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping