PUBLICATION
In situ hybridization patterns of zebrafish homeobox genes homologous to Hox-2.1 and En-2 of mouse
- Authors
- Njolstad, P.R. and Fjose, A.
- ID
- ZDB-PUB-961014-834
- Date
- 1988
- Source
- Biochemical and Biophysical Research Communications 157: 426-432 (Journal)
- Registered Authors
- Fjose, Anders
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Probes
- Fishes/anatomy & histology
- Fishes/genetics*
- Fishes/growth & development
- Genes, Homeobox*
- Nucleic Acid Hybridization
- Sequence Homology, Nucleic Acid
- Transcription, Genetic
- PubMed
- 2904810 Full text @ Biochem. Biophys. Res. Commun.
Citation
Njolstad, P.R. and Fjose, A. (1988) In situ hybridization patterns of zebrafish homeobox genes homologous to Hox-2.1 and En-2 of mouse. Biochemical and Biophysical Research Communications. 157:426-432.
Abstract
Vertebrate homeobox genes have been identified on the basis of sequence homology to Drosophila segmentation and homeotic genes. We have analyzed the spatial distribution patterns in hatching larvae of transcripts from two different types of zebrafish (Brachydanio rerio) homeobox genes. Tritiated DNA fragments from one zebrafish gene (ZF-21) of the Antennapedia-class and one (ZF-EN) of the engrailed-type were used as probes to detect transcripts in larval tissue sections. Both zebrafish genes are expressed mainly in spatially restricted regions of the central nervous system. The transcripts from ZF-21 was detected in the posterior part of the hindbrain, and ZF-EN was found to be most intensively expressed in a restricted domain located in the region of the junction between the mid- and hindbrain. These neural expression patterns are very similar to those of the homologous murine genes Hox-2.1 and En-2 and clearly suggest a strong functional conservation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping