PUBLICATION
Expression of zebrafish nk2.2 is influenced by sonic hedgehog/vertebrate hedgehog-1 and demarcates a zone of neuronal differentiation in the embryonic forebrain
- Authors
- Barth, K.A. and Wilson, S.W.
- ID
- ZDB-PUB-961014-76
- Date
- 1995
- Source
- Development (Cambridge, England) 121: 1755-1768 (Journal)
- Registered Authors
- Barth, Anukampa, Wilson, Steve
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/embryology*
- Cell Differentiation
- Eye/embryology
- Gene Expression Regulation, Developmental*
- Gene Transfer Techniques
- Genes, Homeobox*
- Hedgehog Proteins
- Homeodomain Proteins/genetics*
- In Situ Hybridization
- Molecular Sequence Data
- Neurons/cytology
- Neurons/physiology
- Proteins/genetics*
- Trans-Activators*
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 7600991 Full text @ Development
Citation
Barth, K.A. and Wilson, S.W. (1995) Expression of zebrafish nk2.2 is influenced by sonic hedgehog/vertebrate hedgehog-1 and demarcates a zone of neuronal differentiation in the embryonic forebrain. Development (Cambridge, England). 121:1755-1768.
Abstract
We have isolated zebrafish nk2.2, a member of the Nk-2 family of homeobox genes. nk2.2 is expressed in a continuous narrow band of cells along a boundary zone demarcating the location at which two of the earliest nuclei in the brain differentiate. This band of cells is located within a few cell diameters of cells expressing the signalling molecule sonic hedgehog/vertebrate hedgehog-1 (shh/vhh-1). Injection of shh/vhh-1 RNA results in ectopic expression of nk2.2 and concomitant abnormalities in the forebrain and eyes. Moreover, cyclops mutant embryos, which initially lack neurectodermal expression of shh/vhh-1, show a concomitant lack of nk2.2 expression. Together, these results suggest a requirement of shh/vhh-1 protein for the spatial regulation of nk2.2 expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping