PUBLICATION

Clustering of muscle acetylcholine receptors requires motoneurons in live embryos but not in cell culture

Authors
Liu, D.W. and Westerfield, M.
ID
ZDB-PUB-961014-715
Date
1992
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   12: 1859-1866 (Journal)
Registered Authors
Liu, Dennis, Westerfield, Monte
Keywords
none
MeSH Terms
  • Animals
  • Cells, Cultured
  • Embryo, Nonmammalian/physiology*
  • Motor Neurons/physiology*
  • Muscles/embryology
  • Muscles/innervation
  • Muscles/metabolism*
  • Neuromuscular Junction/physiology
  • Receptor Aggregation/physiology*
  • Receptors, Cholinergic/metabolism*
  • Synaptic Transmission/physiology
  • Tissue Distribution
  • Zebrafish/embryology
PubMed
1315852
Abstract
Previous culture studies have demonstrated that muscle cells autonomously express and cluster ACh receptors (AChRs) and that contact by neurites induces a reorganization of these clusters. We studied these phenomena in zebrafish embryos where the same cells could be examined in vivo and in vitro, and where contacts between cells could be viewed repeatedly. Receptor clusters first appeared when the pioneer growth cones emerged from the spinal cord, were always associated with labeled branches, and developed normally in the presence of neuromuscular transmission blockers. When motoneurons were removed, the muscles failed to cluster receptors. In contrast, muscle cells grown in cell culture uncontacted by nerves clustered AChRs. Our results suggest that clustering of AChRs in living embryos is induced by the presence of neurites and does not occur in the absence of neuronal contact. We suggest that conditions in cell culture, which differ from those in the intact embryo, induce clusters on isolated muscle cells. Moreover, our results demonstrate that receptors cluster without binding transmitter and in the absence of neuronal activity.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes