ZFIN ID: ZDB-PUB-961014-635
Zebrafish pax[zf-a]: a paired box-containing gene expressed in the neural tube
Krauss, S., Johansen, T., Korzh, V., Moens, U., Ericson, J.U., and Fjose, A.
Date: 1991
Source: The EMBO journal   10: 3609-3619 (Journal)
Registered Authors: Fjose, Anders, Johansen, Terje, Korzh, Vladimir, Krauss, Stefan
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA
  • Eye/chemistry
  • Gene Expression
  • Genes, Regulator*
  • Molecular Sequence Data
  • Nervous System/chemistry
  • Nervous System/embryology*
  • Nucleic Acid Hybridization
  • RNA/genetics
  • Rhombencephalon/chemistry
  • Rhombencephalon/embryology
  • Sequence Homology, Nucleic Acid
  • Spinal Cord/chemistry
  • Spinal Cord/embryology
  • Transcription, Genetic
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed: 1718739
Murine and human sequences homologous to the paired box of the Drosophila segmentation gene paired have been reported previously. Here we describe a zebrafish (Brachydanio rerio) paired box-containing clone, pax[zf-a], which is clearly distinct from reported vertebrate Pax genes. The putative protein encoded by pax[zf-a] contains a paired box and a paired-type homeobox separated by a glycine-rich, acidic linker and a carboxy-terminal end which is remarkably rich in serine, threonine and proline residues. By in situ hybridization to embryonic tissue sections and whole mount embryos, pax[zf-a] transcripts were found within restricted regions of the central nervous system and the eye. In contrast to the murine Pax genes recently characterized, pax[zf-a] is not expressed in the segmented mesoderm. At the 17 h stage, pax[zf-a] expression is detected in a defined area of the diencephalon which circumscribes the presumptive thalamus. This suggests an involvement of pax[zf-a] in pattern formation in the rostral brain. The pax[zf-a] gene is also expressed throughout the hindbrain and spinal cord. This hybridization signal is restricted to a longitudinal column which includes the basal plate. Later in development, at 36 h post-fertilization, pax[zf-a] transcripts are no longer restricted to a specific region of the diencephalon, but are distributed over the entire developing brain.