ZFIN ID: ZDB-PUB-961014-633
Expression of the zebrafish paired box gene pax[zf-b] during early neurogenesis
Krauss, S., Johansen, T., Korzh, V., and Fjose, A.
Date: 1991
Source: Development (Cambridge, England)   113(4): 1193-1206 (Journal)
Registered Authors: Fjose, Anders, Johansen, Terje, Korzh, Vladimir, Krauss, Stefan
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Embryo, Nonmammalian/ultrastructure
  • Gene Expression/physiology*
  • Genes/genetics*
  • Kidney/embryology
  • Microscopy, Electron
  • Molecular Probe Techniques
  • Molecular Sequence Data
  • Morphogenesis/genetics*
  • Nervous System/embryology*
  • Nervous System/ultrastructure
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 1811936
The paired box-containing (pax) gene family encodes a group of putative transcription factors differentially expressed during embryonic development. In this study, we describe the cloning and expression of a zebrafish gene pax[zf-b], which most probably is a direct homologue to the mouse Pax2 gene. The putative protein encoded by pax[zf-b] contains a paired box, an octapeptide, but no homeobox. However, a region of homology to the N-terminal half of paired-type homeoboxes is detected C- terminal to the pax[zf-b] paired domain. In zebrafish embryos, pax[zf-b] transcripts are first seen during the formation of the neural keel. At 9-10 h of development, two laterally located transverse stripes of cells expressing the gene appear in the rostral 1/3 of the embryo. The two areas subsequently move towards the midline and form the posterior portion of the midbrain. In the following stages of development, at 10-12 h, transcripts are detected in the otic placode, the Wolffian duct including the nephritic primodium and in the optic stalk. At a later time point, beginning at 14- 15 h, single cells along the spinal cord, presumably interneurons, start to express the gene. The characteristic expression pattern of pax[zf-b] in the neural tube suggests an involvement of this gene in the regionalization of the midbrain as well as in the specification of neuronal cell fates at early embryonic stages.