PUBLICATION
The zebrafish Fgf-3 gene: cDNA sequence, transcript structure and genomic organization
- Authors
- Kiefer, P., Strähle, U., and Dickson, C.
- ID
- ZDB-PUB-961014-565
- Date
- 1996
- Source
- Gene 168(2): 211-215 (Journal)
- Registered Authors
- Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Conserved Sequence
- DNA, Complementary
- Fibroblast Growth Factor 3
- Fibroblast Growth Factors/biosynthesis*
- Fibroblast Growth Factors/genetics*
- Humans
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- Promoter Regions, Genetic*
- Protein-Tyrosine Kinases/biosynthesis
- Protein-Tyrosine Kinases/genetics
- Proto-Oncogene Proteins/biosynthesis*
- Proto-Oncogene Proteins/genetics*
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Transcription, Genetic*
- Xenopus Proteins*
- Xenopus laevis
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 8654946 Full text @ Gene
Citation
Kiefer, P., Strähle, U., and Dickson, C. (1996) The zebrafish Fgf-3 gene: cDNA sequence, transcript structure and genomic organization. Gene. 168(2):211-215.
Abstract
We report the isolation and characterization of genomic and cDNA clones encoding zebrafish fibroblast growth factor 3 (FGF3). An initial cDNA clone was generated by PCR amplification using degenerate oligo primers corresponding to a conserved region of protein found in the mouse and human homologues. Screening a cDNA library made from 30-33-h-old zebrafish embryos with this PCR product led to the isolation of two cDNA clones. Sequence analysis of the longest cDNA insert (1810 bp) revealed a 256-amino-acid (aa) orf. The central region, composed of approx. 155 aa, shares 78% identity with the analogous region of Xenopus laevis FGF3 and 72% identity with the product of the more distantly related human gene. However, the N-and C-terminal domains of zebrafish FGF3 are very different from those of other known homologues. The cDNA was used as a probe on genomic DNA to create a physical map of the locus and to isolate a genomic clone encompassing the entire coding region and 5' sequences. DNA sequencing and RNase protection analyses indicate that zebrafish Fgf-3 (ZFgf-3) is structurally analogous to the mouse gene and regulated through two different promoters. The transcription start point of the proximal promoter aligns to that of mouse promoter P3 and lies within a conserved region of sequence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping