ZFIN ID: ZDB-PUB-961014-480
Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish
Inoue, A., Takahashi, M., Hatta, K., Hotta, Y., and Okamoto, H.
Date: 1994
Source: Developmental dynamics : an official publication of the American Association of Anatomists   199: 1-11 (Journal)
Registered Authors: Hatta, Kohei, Okamoto, Hitoshi
Keywords: Zebrafish, Isl-1 cDNA, Primary motoneurons, Neuronal identity, Zebrafish mutant
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/embryology
  • Brain/metabolism
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Conserved Sequence
  • DNA-Binding Proteins/biosynthesis*
  • DNA-Binding Proteins/chemistry
  • DNA-Binding Proteins/genetics
  • Homeodomain Proteins*
  • LIM-Homeodomain Proteins
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Neurons/physiology*
  • RNA, Messenger/biosynthesis*
  • Rats
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Transcription Factors/biosynthesis*
  • Transcription Factors/chemistry
  • Transcription Factors/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 8167375 Full text @ Dev. Dyn.
Islet-1 (Isl-1) is a LIM domain/homeodomain-type transcription regulator that has been originally identified as an insulin gene enhancer binding protein. Isl-1 is also expressed by subsets of neurons in the central nervous system of rat and chick embryos. We have cloned the Isl-1 cDNA from zebrafish and examined its expression pattern using in situ hybridization to whole-mount embryos. Isl-1 mRNA first appears immediately after gastrulation in the polster, the cranial ganglia, and in Rohon-Beard neurons and ventromedial cells of the spinal cord. The expression by the ventromedial cells is segmentally repeated and becomes restricted to the one or two cells slightly anterior to the segment borders. Double staining by in situ hybridization and an antibody which stains most axons suggested that these segmentally distributed cells may be either the rostral primary motoneuron (RoP) or middle primary motoneuron (MiP). This raises a possibility that Isl-1 may be involved during determination of subtype identities of the primary motoneurons. Furthermore, the specific Isl-1 mRNA expression in the spinal cord is under the control of the somites, since mutant embryo with defective somite failed to maintain this pattern.