PUBLICATION

Commitment of cell fate in the early zebrafish embryo

Authors
Ho, R.K. and Kimmel, C.B.
ID
ZDB-PUB-961014-461
Date
1993
Source
Science (New York, N.Y.)   261: 109-111 (Journal)
Registered Authors
Ho, Robert K., Kimmel, Charles B.
Keywords
none
MeSH Terms
  • Animals
  • Blastocyst/cytology*
  • Blastoderm/cytology*
  • Cell Movement
  • Gastrula/cytology*
  • Stem Cell Transplantation
  • Stem Cells/cytology*
  • Transplantation, Heterotopic
  • Zebrafish/embryology*
PubMed
8316841 Full text @ Science
Abstract
When do single cells in the early zebrafish embryo become irreversibly committed to a specific fate? Work with lineage tracing and fate mapping has shown that the marginal cells of the blastoderm give rise to hypoblast-derived fates (mesoderm and endoderm). However, experiments described here show that these marginal blastoderm cells remain pluripotent and uncommitted throughout the late blastula and early gastrula stages. Embryonic cells become committed to a hypoblast-derived fate at mid-gastrulation. Time-lapse photographic analysis reveals that committed cells, when transplanted heterotopically and heterochronically, can migrate along atypical pathways to reposition themselves within a more correct environment.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping