PUBLICATION
Induction of recessive lethal mutations in zebrafish with ultraviolet light
- Authors
- Grunwald, D.J., and Streisinger, G.
- ID
- ZDB-PUB-961014-378
- Date
- 1992
- Source
- Genetics research 59: 93-101 (Journal)
- Registered Authors
- Grunwald, David, Streisinger, George
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Damage
- Female
- Fertility/radiation effects
- Genes, Lethal
- Genotype
- Male
- Mosaicism
- Mutagenesis
- Mutagenesis, Site-Directed
- Phenotype
- Spermatozoa/radiation effects*
- Ultraviolet Rays
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 1628821 Full text @ Genet. Res.
Citation
Grunwald, D.J., and Streisinger, G. (1992) Induction of recessive lethal mutations in zebrafish with ultraviolet light. Genetics research. 59:93-101.
Abstract
Recessive lethal germline and specific locus somatic mutations were induced efficiently in the zebrafish by exposure of mature sperm to UV light. Mutagenesis of sperm yielded mosaic individuals: clones bearing novel mutations represented approximately 12-25% of the haploid germ cells and 25-50% of the somatic tissue. Simple methods are described for the reliable identification and propagation of newly arising developmental mutations in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping