PUBLICATION

Embryonic expression and DNA-binding properties of zebrafish pax-6

Authors
Amirthalingam, K., Lorens, J.B., Sætre, B.O., Salaneck, E., and Fjose, A.
ID
ZDB-PUB-961014-35
Date
1995
Source
Biochemical and Biophysical Research Communications   215: 122-128 (Journal)
Registered Authors
Fjose, Anders, Lorens, James B.
Keywords
none
MeSH Terms
  • Animals
  • DNA/metabolism*
  • DNA Probes
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism*
  • Eye/embryology
  • Eye/metabolism
  • Eye Proteins
  • Gene Expression*
  • Homeodomain Proteins*
  • In Situ Hybridization
  • Nervous System/embryology
  • Nervous System/metabolism
  • Paired Box Transcription Factors
  • Polymerase Chain Reaction
  • Prosencephalon/embryology
  • Prosencephalon/metabolism
  • RNA, Messenger/metabolism
  • Repressor Proteins
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism
  • Transcription Factors*
  • Zebrafish/embryology*
PubMed
7575578 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Zebrafish pax-6 (pax[zf-a]) and its murine homologue are structurally and functionally related to the Drosophila paired box gene eyeless, a master control gene for eye development. This report details the zebrafish pax-6 embryonic expression pattern both at the mRNA and protein level. Transcripts are first detected in the presumptive forebrain and hindbrain regions of the neural plate. After formation of the neural keel, Pax-6 protein accumulates within the same two domains. Expression is also observed in the optic vesicles and lens placodes, confirming that the Pax-6 protein is expressed in those areas of the eye where it is assumed to control differentiation. The relative DNA-binding affinity of the zebrafish Pax-6 protein to different categories of Pax recognition sites is shared with the murine homologue.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping