ZFIN ID: ZDB-PUB-961014-346
Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos
Ghosh, C. and Collodi, P.
Date: 1994
Source: Cytotechnology   14: 21-26 (Journal)
Registered Authors: Collodi, Paul, Ghosh, Chandramallika
Keywords: none
MeSH Terms:
  • Animals
  • Blastocyst/cytology*
  • Cell Division
  • Cells, Cultured*
  • Culture Media
  • Zebrafish/embryology*
PubMed: 7765109 Full text @ Cytotechnology
The zebrafish has become a popular model for studies of vertebrate development and toxicology. However, in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum.