PUBLICATION
Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos
- Authors
- Ghosh, C. and Collodi, P.
- ID
- ZDB-PUB-961014-346
- Date
- 1994
- Source
- Cytotechnology 14: 21-26 (Journal)
- Registered Authors
- Collodi, Paul, Ghosh, Chandramallika
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastocyst/cytology*
- Cell Division
- Cells, Cultured*
- Culture Media
- Zebrafish/embryology*
- PubMed
- 7765109 Full text @ Cytotechnology
Citation
Ghosh, C. and Collodi, P. (1994) Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos. Cytotechnology. 14:21-26.
Abstract
The zebrafish has become a popular model for studies of vertebrate development and toxicology. However, in vitro approaches utilizing this organism have not been fully exploited due to the absence of suitable cell culture systems. Previously, we developed methods for the culture of cells derived from zebrafish blastula-stage embryos. One of these cultures, ZEM-2, was derived in a complex medium containing trout embryo extract, trout serum and medium conditioned by buffalo rat liver cells. In this study we describe a zebrafish embryo cell line, ZEM-2A, derived from ZEM-2 following selection for growth in a simplified medium. Optimal growth of ZEM-2A cells is attained in nutrient medium supplemented with 5% fetal bovine serum.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping