PUBLICATION

Identification of two families of satellite-like repetitive DNA sequences from the zebrafish (Brachydanio rerio)

Authors
Ekker, M., Fritz, A., and Westerfield, M.
ID
ZDB-PUB-961014-281
Date
1992
Source
Genomics   13: 1169-1173 (Journal)
Registered Authors
Ekker, Marc, Fritz, Andreas, Westerfield, Monte
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Satellite/genetics*
  • Genomic Library
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid*
  • Zebrafish/genetics*
PubMed
1339388 Full text @ Genomics
Abstract
To further our understanding of the structure and organization of the zebrafish genome, we have undertaken the analysis of highly and middle-repetitive DNA sequences. We have cloned and sequenced two families of tandemly repeated DNA fragments. The monomer units of the Type I satellite-like sequence are 186 bp long, A+T-rich (65%), and exhibit a high degree of sequence conservation. The Type I satellite-like sequence constitutes 8% of the zebrafish genome, or approximately 8 x 10(5) copies per haploid genome. Southern analysis of genomic DNA, digested with several restriction endonucleases, shows a ladder of hybridizing bands, consistent with a tandem array, and suggests longer range periodic variations in the sequence of the tandem repeats. The Type II satellite has a monomer length of 165 bp, is also A+T-rich (68%), and constitutes 0.2% of the zebrafish genome (22,000 copies per haploid genome). Southern analysis reveals a complex pattern rather than a ladder of regularly spaced hybridizing bands.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes