PUBLICATION
Voltage- and Ca2+-gated currents in zebrafish olfactory receptor neurons
- Authors
- Corotto, F.S., Piper, D.R., Chen, N.S., and Michel, W.C.
- ID
- ZDB-PUB-961014-187
- Date
- 1996
- Source
- The Journal of experimental biology 199: 1115-1126 (Journal)
- Registered Authors
- Michel, William C.
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/pharmacology
- Calcium Channels/physiology*
- Cobalt/pharmacology
- Electric Conductivity
- Ion Channel Gating/physiology*
- Olfactory Receptor Neurons/physiology*
- Potassium Channels/physiology
- Tetrodotoxin/pharmacology
- Zebrafish/physiology*
- PubMed
- 8786333 Full text @ J. Exp. Biol.
Citation
Corotto, F.S., Piper, D.R., Chen, N.S., and Michel, W.C. (1996) Voltage- and Ca2+-gated currents in zebrafish olfactory receptor neurons. The Journal of experimental biology. 199:1115-1126.
Abstract
Voltage- and Ca(2+)-gated currents were recorded from isolated olfactory receptor neurons (ORNs) of the zebrafish Danio rerio using the whole-cell voltage-clamp technique. Zebrafish ORNs had an average capacitance of 0.66 pF and an average apparent input resistance of 8.0 G omega. Depolarizing steps elicited transient inward currents followed by outward currents with transient and sustained components. The transient inward current (INa) was sensitive to 1 mumol l-1 tetrodotoxin, activated between -74mV and -64mV, and reached half-maximal conductance at -28 mV. Its peak amplitude averaged -101pA. Steady-state inactivation of INa was half-maximal at an average test potential of -78mV and recovery from inactivation proceeded with two time constants averaging 23 ms and 532 ms. A sustained, Co(2+)-sensitive current (ICa) activated between - 44mV and -34mV and reached a peak amplitude averaging -9pA at - 14mV. Outward currents were carried by K+, based on the reversal potentials of tail currents, and consisted of a Ca(2+)-dependent K+ current, a delayed rectifier current (IDR) and a transient K+ current (IA). The Ca(2+)-dependent K+ current (IK(Ca)) activated between -44mV and -34mV, whereas IDR and IA activated between -34mV and -24mV. In summary, zebrafish ORNs possess a complement of gated currents similar but not identical to that of ORNs from other vertebrates and which appears well suited for encoding a graded receptor potential into a train of action potentials.
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