PUBLICATION
Production of androgenetic zebrafish (Danio rerio)
- Authors
- Corley-Smith, G.E., Lim, C.J.T., and Brandhorst, B.P.
- ID
- ZDB-PUB-961014-186
- Date
- 1996
- Source
- Genetics 142: 1265-1276 (Journal)
- Registered Authors
- Brandhorst, Bruce P., Corley-Smith, Graham, Lim, Chinten James
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- DNA Primers
- Diploidy
- Female
- Genetic Markers
- Genomic Imprinting
- Haploidy
- Major Histocompatibility Complex
- Male
- Molecular Sequence Data
- Parthenogenesis
- Pedigree
- Polymerase Chain Reaction
- Radiation Dosage
- Random Amplified Polymorphic DNA Technique
- Repetitive Sequences, Nucleic Acid
- Zebrafish/genetics*
- PubMed
- 8846903 Full text @ Genetics
Citation
Corley-Smith, G.E., Lim, C.J.T., and Brandhorst, B.P. (1996) Production of androgenetic zebrafish (Danio rerio). Genetics. 142:1265-1276.
Abstract
To help investigate the evolutionary origin of the imprinting (parent-of-origin mono-allelic expression) of paternal genes observed in mammals, we constructed haploid and diploid androgenetic zebrafish (Danio rerio). Haploid androgenotes were produced by fertilizing eggs that had been X- ray irradiated to eliminate the maternal genome. Subsequent inhibition of the first mitotic division of haploid androgenotes by heat shock produced diploid androgenotes. The lack of inheritance of maternal-specific DNA markers (RAPD and SSR) by putative diploid and haploid androgenotes confirmed the androgenetic origin of their genomes. Marker analysis was performed on 18 putative androgenotes (five diploids and 13 haploids) from six families. None of 157 maternal-specific RAPD markers analyzed, some of which were apparently homozygous, were passed on to any of these putative androgenotes. A mean of 7.7 maternal-specific markers were assessed per family. The survival of androgenetic zebrafish suggests that if paternal imprinting occurs in zebrafish, it does not result in essential genes being inactivated when their expression is required for development. Production of haploid androgenotes can be used to determine the meiotic recombination rate in male zebrafish. Androgenesis may also provide useful information about the mechanism of sex determination in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping