PUBLICATION

Induction of zebrafish (Brachydanio rerio) P450 in vivo and in cell culture

Authors
Collodi, P., Miranda, C.L., Zhao, X., Buhler, D.R., and Barnes, D.W.
ID
ZDB-PUB-961014-181
Date
1994
Source
Xenobiotica; the fate of foreign compounds in biological systems   24: 487-493 (Journal)
Registered Authors
Barnes, David W., Buhler, Donald R., Collodi, Paul
Keywords
none
MeSH Terms
  • Animals
  • Aryl Hydrocarbon Hydroxylases/biosynthesis
  • Aryl Hydrocarbon Hydroxylases/metabolism
  • Blotting, Western
  • Cells, Cultured
  • Cytochrome P-450 CYP1A1
  • Cytochrome P-450 Enzyme System/biosynthesis*
  • Cytochrome P-450 Enzyme System/immunology
  • Cytochrome P-450 Enzyme System/metabolism
  • Diploidy
  • Embryo, Nonmammalian
  • Enzyme Induction/drug effects
  • Haploidy
  • Liver/drug effects
  • Liver/enzymology
  • Oxidoreductases/biosynthesis
  • Oxidoreductases/metabolism
  • Subcellular Fractions/enzymology
  • Zebrafish/metabolism*
PubMed
7975714 Full text @ Xenobiotica
Abstract
1. Induction of zebrafish P450 by 2,3,7,8-tetrachloro- dibenzo-p-dioxin (TCDD) was studied in liver tissue, primary liver cell culture and multipassage cell culture derived from zebrafish haploid and diploid embryos and liver. 2. TCDD induced two hepatic proteins (54 and 50 kDa) in vivo which were recognized by anti-trout P4501A1 IgG. The 54-kDa protein was induced by TCDD in primary and multipassage hepatocyte cultures and in haploid and diploid embryo-derived cells. The proteins in liver homogenates were not induced by aqueous exposure of zebrafish to beta-naphthoflavone (BNF). 3. Homogenates of zebrafish liver, cultured hepatocytes and embryo- derived cells also exhibited increased ethoxyresorufin O- deethylase (EROD) and 7-12-dimethyl-benz[a]anthracene (DMBA) hydroxylase activity following TCDD exposure.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping