PUBLICATION
Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues
- Authors
- Collodi, P., Kamei, Y., Ernst, T., Miranda, C., Buhler, D.R., and Barnes, D.W.
- ID
- ZDB-PUB-961014-179
- Date
- 1992
- Source
- Cell biology and toxicology 8(1): 43-61 (Journal)
- Registered Authors
- Barnes, David W., Buhler, Donald R., Collodi, Paul
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Division
- Cells, Cultured
- Culture Media
- Cytochrome P-450 CYP1A1
- Cytochrome P-450 Enzyme System/metabolism
- Embryonic and Fetal Development/drug effects
- Enzyme Induction/drug effects
- Haploidy
- Karyotyping
- Oxidoreductases/metabolism
- Plasmids/genetics
- Transfection/genetics
- Zebrafish*
- PubMed
- 1591622 Full text @ Cell Biol. Toxicol.
Citation
Collodi, P., Kamei, Y., Ernst, T., Miranda, C., Buhler, D.R., and Barnes, D.W. (1992) Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell biology and toxicology. 8(1):43-61.
Abstract
The zebrafish is a popular model for studies of vertebrate development and toxicology. However, in vitro approaches with this organism have not been fully exploited because cell culture systems have been unavailable. We developed methods for the culture of cells from blastula-stage diploid and haploid zebrafish embryos, as well as cells from the caudal and pelvic fin, gill, liver, and viscera of adult fish. The haploid embryo-derived cells differentiated in culture to a pigmented phenotype and expressed, upon exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin, a protein that was immunologically and functionally similar to rainbow trout cytochrome P450IA1. Zebrafish cultures were grown in a complex basal nutrient medium supplemented with insulin, trout embryo extract, and low concentrations of trout and fetal bovine serum; they could not be maintained in conventional culture medium containing a high concentration of mammalian serum. Using calcium phosphate-mediated transfection, a plasmid constructed for use in mammalian cells was introduced into zebrafish embryo cell cultures and expressed in a stable manner. These results indicated that the transfection procedures utilized in mammalian systems can also be applied to zebrafish cell cultures, providing a means for in vitro alteration of the genotype and phenotype of the cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping