PUBLICATION
Ontogeny of odorant receptor gene expression in zebrafish, Danio rerio
- Authors
- Byrd, C.A., Jones, J.T., Quattro, J.M., Rogers, M.E., Brunjes, P.C., and Vogt, R.G.
- ID
- ZDB-PUB-961014-154
- Date
- 1996
- Source
- Journal of neurobiology 29: 445-458 (Journal)
- Registered Authors
- Brunjes, Peter C., Byrd-Jacobs, Christine, Quattro, Joseph M., Rogers, Matthew, Vogt, Richard
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- Female
- Gene Expression Regulation, Developmental/physiology
- In Situ Hybridization
- Male
- Molecular Sequence Data
- Neurons, Afferent/chemistry
- Neurons, Afferent/physiology
- Phylogeny
- Receptors, Odorant/genetics*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 8656210 Full text @ J. Neurobiol.
Citation
Byrd, C.A., Jones, J.T., Quattro, J.M., Rogers, M.E., Brunjes, P.C., and Vogt, R.G. (1996) Ontogeny of odorant receptor gene expression in zebrafish, Danio rerio. Journal of neurobiology. 29:445-458.
Abstract
We cloned three putative odorant receptor (OR) genes from the zebrafish to use as in situ hybridization probes to follow the temporal patterns of neurons expressing OR genes through a developmental progression from embryo (12 h postfertilization) to adult. The identification of these genes is supported by sequence homology to previously reported ORs and by the morphology and location of labeled cells in in situ hybridization experiments. Cells expressing OR mRNA were first observed in the olfactory placodes between 31 and 38 h after fertilization (fish reared at 26 degrees C). Initially, only single cells were observed to hybridize the probe; the number of labeled cells increased throughout the remainder of embryogenesis and through postembryonic growth and morphogenesis of the olfactory organ. At all ages, the positively hybridizing cells were scattered throughout the olfactory epithelium but not in the nonsensory epithelium of the olfactory organ.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping