PUBLICATION
The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles
- Authors
- Zelenin, A.V., Alimov, A.A., Barmintzev, V.A., Benyumov, A.O., Zelenina, I.A., Krasnov, A.M., and Kolesnikov, V.A.
- ID
- ZDB-PUB-961014-1296
- Date
- 1991
- Source
- FEBS letters 287: 118-120 (Abstract)
- Registered Authors
- Benyumov, Alexey O.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cypriniformes/embryology*
- DNA/analysis
- Gene Expression
- Kanamycin Kinase
- Larva/analysis
- Microspheres
- Phosphotransferases/genetics
- Plasmids
- Polymerase Chain Reaction
- Transfection*
- Trout/embryology*
- Tungsten
- Zebrafish/embryology*
- Zygote*
- beta-Galactosidase/genetics
- PubMed
- 1652457 Full text @ FEBS Lett.
Citation
Zelenin, A.V., Alimov, A.A., Barmintzev, V.A., Benyumov, A.O., Zelenina, I.A., Krasnov, A.M., and Kolesnikov, V.A. (1991) The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles. FEBS letters. 287:118-120.
Abstract
Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of beta-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping