PUBLICATION
A zebrafish homologue of the Drosophila neurogenic gene Notch and its pattern of transcription during early embryogenesis
- Authors
- Bierkamp, C. and Campos-Ortega, J.A.
- ID
- ZDB-PUB-961014-104
- Date
- 1993
- Source
- Mechanisms of Development 43: 87-100 (Journal)
- Registered Authors
- Bierkamp, Christiane, Campos-Ortega, Jose
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Brain/embryology
- Brain/metabolism
- Cloning, Molecular
- DNA, Complementary/isolation & purification
- Drosophila/embryology
- Drosophila/genetics*
- Embryonic Development
- Mesoderm/metabolism
- Molecular Sequence Data
- Nervous System/embryology*
- RNA, Messenger/analysis
- Sequence Homology, Amino Acid
- Time Factors
- Transcription, Genetic*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 8297791 Full text @ Mech. Dev.
Citation
Bierkamp, C. and Campos-Ortega, J.A. (1993) A zebrafish homologue of the Drosophila neurogenic gene Notch and its pattern of transcription during early embryogenesis. Mechanisms of Development. 43:87-100.
Abstract
We describe here the primary structure of a zebrafish homologue of the Drosophila neurogenic gene Notch and its pattern of mRNA accumulation during embryogenesis. The gene produces a 8.5 kb transcript encoding a putative transmembrane protein with a high degree of sequence similarity to members of the Notch family, comprising 36 EGF-like repeats, three lin- 12/Notch repeats, six cdc10/SW16 repeats, OPA repeats and a PEST sequence. Transcription of the zebrafish Notch gene is spatially and temporally regulated. A high density of transcripts, most probably of maternal origin, can already be detected in the 2-cell stage. During pregastrulation stages, RNA is present in all cells. However, following gastrulation, transcripts accumulate in specific regions of the embryo following a rapidly changing pattern. In some of these regions, cell divisions take place at the time of Notch expression, in others processes of cell differentiation. This holds true for various mesodermal derivatives, such as the prospective notochord, and for different neural primordia, such as the neural plate and the brain vesicles. This pattern of transcript accumulation suggests a role for the zebrafish Notch homologue in processes of regionalization and cell diversification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping