ZFIN ID: ZDB-PUB-961014-1008
The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo
Schulte-Merker, S., Ho, R.K., Herrmann, B.G., and Nüsslein-Volhard, C.
Date: 1992
Source: Development (Cambridge, England)   116: 1021-1032 (Journal)
Registered Authors: Ho, Robert K., Nüsslein-Volhard, Christiane, Schulte-Merker, Stefan
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Embryonic Induction/genetics*
  • Gene Expression/physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Mesoderm/physiology*
  • Mice
  • Molecular Sequence Data
  • Notochord/physiology*
  • Nuclear Proteins/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 1295726
Embryos mutant for the T gene, in mice, make insufficient mesoderm and fail to develop a notochord. We report the cloning and sequencing of the T gene in the zebrafish (Brachydanio rerio) and show the nuclear localization of the protein product. Both RNA and protein are found in cells of the germ ring, including enveloping layer cells, prior to and during gastrulation of zebrafish embryos. Nuclei of the yolk syncytial layer do not express Zf-T. High levels of expression are maintained throughout early development in the notochord, while in paraxial mesoderm cells the gene is turned off during gastrulation. Exposure of animal cap cells to activinA induces Zf-T expression, as does transplantation into the germ ring.