PUBLICATION
The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish
- Authors
- Sugitani, K., Omori, Y., Mokuya, T., Hosoi, S., Kobayashi, H., Miyata, K., Araiso, Y., Koriyama, Y.
- ID
- ZDB-PUB-250814-18
- Date
- 2025
- Source
- International Journal of Molecular Sciences 26: (Journal)
- Registered Authors
- Araiso, Yuhei
- Keywords
- Bcl-2, CRISPR/Cas9 system, HSF1, MYDGF, TNFα, caspase-3, optic nerve regeneration, retina, zebrafish: cell survival
- MeSH Terms
-
- Retina*/metabolism
- Retina*/physiology
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Cell Survival
- Intercellular Signaling Peptides and Proteins*/genetics
- Intercellular Signaling Peptides and Proteins*/metabolism
- Optic Nerve Injuries/genetics
- Optic Nerve Injuries/metabolism
- Apoptosis
- Regeneration*
- Nerve Regeneration*
- Zebrafish/genetics
- Zebrafish/metabolism
- Animals
- PubMed
- 40806387 Full text @ Int. J. Mol. Sci.
Citation
Sugitani, K., Omori, Y., Mokuya, T., Hosoi, S., Kobayashi, H., Miyata, K., Araiso, Y., Koriyama, Y. (2025) The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish. International Journal of Molecular Sciences. 26:.
Abstract
Myeloid-derived growth factor (MYDGF), named in reference to its secretion from myeloid cells in bone marrow, is a novel protein with anti-apoptotic and tissue-repairing properties. MYDGF is found in various human tissues affected by different diseases. To date, however, MYDGF expression has yet to be reported in the nervous system. Herein, we demonstrate for the first time that MYDGF mRNA levels increased in the zebrafish retina 1 h after optic nerve injury (ONI). MYDGF-producing cells were located in the photoreceptors and infiltrating leukocytic cells. We prepared the retina for MYDGF gene knockdown by performing intraocular injections using either MYDGF-specific morpholino or the CRISPR/Cas9 system. Under these MYDGF-knockdown retinal conditions, anti-apoptotic Bcl-2 mRNA was suppressed; in comparison, apoptotic caspase-3 and inflammatory TNFα mRNA were significantly upregulated in the zebrafish retina after ONI compared to the control. Furthermore, heat shock factor 1 (HSF1) was evidently suppressed under these conditions, leading to a significant number of apoptotic neurons. These findings indicate that MYDGF is a key molecule in the stimulation of neuronal regeneration in the central nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping