PUBLICATION
Elf1 Deficiency Impairs Macrophage Development in Zebrafish Model Organism
- Authors
- Tan, Q., Wang, J., Hao, Y., Yang, S., Cao, B., Pan, W., Cao, M.
- ID
- ZDB-PUB-250327-20
- Date
- 2025
- Source
- International Journal of Molecular Sciences 26: (Journal)
- Registered Authors
- Pan, Weijun
- Keywords
- ELF1, ETS, macrophage, zebrafish
- MeSH Terms
-
- Receptors, CXCR4/genetics
- Receptors, CXCR4/metabolism
- Gene Knockdown Techniques
- Cell Differentiation/genetics
- CRISPR-Cas Systems
- Animals
- Macrophages*/metabolism
- Zebrafish*/genetics
- Zebrafish Proteins*/deficiency
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Gene Editing
- Transcription Factors/deficiency
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Cell Movement/genetics
- PubMed
- 40141178 Full text @ Int. J. Mol. Sci.
Citation
Tan, Q., Wang, J., Hao, Y., Yang, S., Cao, B., Pan, W., Cao, M. (2025) Elf1 Deficiency Impairs Macrophage Development in Zebrafish Model Organism. International Journal of Molecular Sciences. 26:.
Abstract
The Ets (E-twenty-six) family of transcription factors plays a critical role in hematopoiesis and myeloid differentiation. However, the specific functions of many family members in these processes remain largely underexplored and poorly understood. Here, we identify Elf1 (E74-like factor 1), an Ets family member, as a critical regulator of macrophage development in the zebrafish model organism, with minimal impact on neutrophil differentiation. Through morpholino knockdown screening and CRISPR/Cas9-mediated gene editing, we demonstrate that Elf1 is critical for macrophage development and tissue injury responses. Specific overexpression of dominant-negative Elf1 (DN-Elf1) in macrophages demonstrated a cell-autonomous effect on macrophage infiltration. Furthermore, the overexpression of cxcr4b, a gene downstream of Elf1 regulation and essential for cell migration and injury response, significantly rescued this defect, indicating Elf1 as a key regulator of macrophage function. Our findings shed light on the roles of Elf1 in macrophage development and injury response and also highlight zebrafish as a powerful model for immunity research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping