PUBLICATION
PIKFYVE deficiency induces vacuole-like cataract via perturbing late endosome homeostasis
- Authors
- Ma, X., Yu, S., Zhang, M., Mei, S., Ling, Y., Huang, X., Dong, S., Fan, B., Zhao, J.
- ID
- ZDB-PUB-250109-205
- Date
- 2024
- Source
- Biochemical and Biophysical Research Communications 747: 151123151123 (Journal)
- Registered Authors
- Keywords
- Late endosome homeostasis, Lens, PIKFYVE, Proteomics, Transcriptomics, Vacuole
- MeSH Terms
-
- Epithelial Cells/metabolism
- Epithelial Cells/pathology
- Cataract*/genetics
- Cataract*/metabolism
- Cataract*/pathology
- Homeostasis*
- Vacuoles*/metabolism
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/metabolism
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Animals
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Androstenes
- Humans
- rab7 GTP-Binding Proteins/metabolism
- Lens, Crystalline*/metabolism
- Lens, Crystalline*/pathology
- Endosomes*/metabolism
- Macrolides/pharmacology
- Phosphatidylinositol 3-Kinases*/metabolism
- PubMed
- 39778216 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ma, X., Yu, S., Zhang, M., Mei, S., Ling, Y., Huang, X., Dong, S., Fan, B., Zhao, J. (2024) PIKFYVE deficiency induces vacuole-like cataract via perturbing late endosome homeostasis. Biochemical and Biophysical Research Communications. 747:151123151123.
Abstract
Phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE) was recently identified as a causative gene for cataract. Pikfyve phosphatidylinositol phosphate kinase domain-deficient (pikfyveΔ8) zebrafish lens and PIKFYVE-inhibited human lens epithelial cells developed vacuoles, colocalized with late endosome marker RAB7. In this study, the pikfyveΔ8zebrafish with vacuole-like cataract underwent transcriptomic and proteomic analyses to explore the underlying mechanisms of vacuole formation. PIKFYVE-knockout and PIKFYVE-inhibited human lens epithelial cells with vacuoles further verified these omics results and rescued with Bafilomycin A1(Baf-A1) and U18666A. We discovered no significant differences in lysosomal fusion, but upregulation in acid hydrolase. The composition of late endosomal membrane was changed, and vacuolar ATPase and endosomal sorting complexes required for transport (ESCRT) at late endosome were upregulated. These changes are related with the late endosome homeostasis. Strikingly, vacuoles in human lens epithelial cells could be partially rescued by Baf-A1 and almost completely rescued by U18666A. Collectively, these findings suggest that vacuoles in pikfyveΔ8 zebrafish lens and PIKFYVE-inhibited cells were colocalized with swollen late endosomes, and generated by perturbing late endosome homeostasis due to enhanced ESCRT mechanisms and decreased stability in late endosomal membrane. This study expands our understanding of the mechanisms underlying cataract development and reveals potentially effective therapeutic targets.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping