PUBLICATION
Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis
- Authors
- Mori, Y., Smith, S., Wang, J., Eliora, N., Heikes, K.L., Munjal, A.
- ID
- ZDB-PUB-241210-4
- Date
- 2024
- Source
- Development (Cambridge, England) 152(1): (Journal)
- Registered Authors
- Munjal, Akankshi
- Keywords
- ECM, Inner ear semicircular canals, Lmx1b, Tissue morphogenesis, Versican-hyaluronan, Zebrafish development
- MeSH Terms
-
- Morphogenesis*
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Hyaluronic Acid*/metabolism
- Animals
- Semicircular Canals*/embryology
- Semicircular Canals*/metabolism
- Gene Expression Regulation, Developmental*
- Zebrafish*/embryology
- Zebrafish*/genetics
- Versicans*/genetics
- Versicans*/metabolism
- Transcription Factors*/genetics
- Transcription Factors*/metabolism
- Extracellular Matrix/metabolism
- PubMed
- 39651757 Full text @ Development
Citation
Mori, Y., Smith, S., Wang, J., Eliora, N., Heikes, K.L., Munjal, A. (2024) Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis. Development (Cambridge, England). 152(1):.
Abstract
During inner ear semicircular canal morphogenesis in zebrafish, patterned canal-genesis zones express genes for extracellular matrix component synthesis. These include hyaluronan and the hyaluronan-binding chondroitin sulfate proteoglycan Versican, which are abundant in the matrices of many developing organs. Charged hyaluronate polymers play a key role in canal morphogenesis through osmotic swelling. However, the developmental factor(s) that pattern the synthesis of the matrix components and regulation of hyaluronate density and swelling are unknown. Here, we identify the transcription factor, Lmx1b, as a positive transcriptional regulator of hyaluronan, Versican, and chondroitin synthesis genes crucial for canal morphogenesis. We show that Versican regulates hyaluronan density through its protein core, whereas the charged chondroitin side chains contribute to the hydration of hyaluronate-ECM. Versican-tuned properties of hyaluronate matrices may be a broadly used mechanism in morphogenesis with important implications for understanding diseases where these matrices are impaired, and for hydrogel engineering for tissue regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping