PUBLICATION
Dynamic gene expression pattern in zebrafish gall bladder - an experimental study
- Authors
- Tanwar, P., Bhattacharya, D., Dasari, A., Bijwe, M., Rana, R., Gupta, I., Minocha, S.
- ID
- ZDB-PUB-241203-2
- Date
- 2024
- Source
- International journal of surgery (London, England) 110(12): 7585-7589 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Zebrafish*/genetics
- Gallbladder Neoplasms*/genetics
- Gallbladder Neoplasms*/metabolism
- Gallbladder Neoplasms*/pathology
- Animals
- Disease Models, Animal
- Transcriptome
- Gene Expression Profiling
- Humans
- Gallbladder*/metabolism
- Gallbladder*/pathology
- PubMed
- 39621022 Full text @ Int J Surg
Citation
Tanwar, P., Bhattacharya, D., Dasari, A., Bijwe, M., Rana, R., Gupta, I., Minocha, S. (2024) Dynamic gene expression pattern in zebrafish gall bladder - an experimental study. International journal of surgery (London, England). 110(12):7585-7589.
Abstract
Gall bladder cancer (GBC) is one of the common biliary tract malignancies that is often associated with late presentation of clinical symptoms resulting in poor prognosis, thereby considerably increasing the mortality rate. Geographic and ethnic variations are one of the major causes of GBC incidence. However, in addition to genetic susceptibility to GBC, other factors like age, obesity, gender, and exposure to certain heavy chemicals may also lead to the incidence of GBC. Most of GBC research has so far used traditional cell culture systems. Recently, newer approaches using 2D and 3D cultures, xenograft models, and organoids are also becoming popular. However, whole organism model systems for GBC research have not yet been established. In this study, we propose the use of zebrafish as a model organism to study GBC. However, the zebrafish gall bladder is relatively unexplored besides the anatomical features. Here, we unravel and then compare the unique transcriptomic profile of zebrafish gall bladder with humans to identify genes consistently expressed in both species. We provide a comprehensive list of all gall bladder specific genes in zebrafish that also have their orthologous counterparts in humans. Therefore, they can be used as potential biomarkers for gall bladder transcriptomic profile. To the best of our knowledge, this is the only study so far to provide a complete genomic expression profile of zebrafish gall bladder along with an exhaustive cross-species comparison, thus paving the path for using zebrafish as a model organism to study GBC and help identify novel biomarkers for its early detection and diagnosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping