PUBLICATION

The embryonic zebrafish brain is exclusively colonized by pu.1-dependent and lymphatic-independent population of microglia

Authors
Yu, T., Chen, J., Wang, Y., Xu, J.
ID
ZDB-PUB-240829-7
Date
2024
Source
Science advances   10: eado0519 (Journal)
Registered Authors
Xu, Jin, YU, Tao
Keywords
none
MeSH Terms
  • Proto-Oncogene Proteins*/genetics
  • Proto-Oncogene Proteins*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Trans-Activators*/genetics
  • Trans-Activators*/metabolism
  • Microglia*/metabolism
  • Brain*/embryology
  • Brain*/metabolism
  • Zebrafish*/embryology
  • Embryo, Nonmammalian/metabolism
  • Lymphatic Vessels*/cytology
  • Lymphatic Vessels*/embryology
  • Lymphatic Vessels*/metabolism
  • Animals
(all 15)
PubMed
39196933 Full text @ Sci Adv
Abstract
Microglia, the crucial immune cells inhabiting the central nervous system (CNS), perform a range of vital functions, encompassing immune defense and neuronal regulation. Microglia subsets with diverse functions and distinct developmental regulations have been identified recently. It is generally accepted that all microglia originate from hematopoiesis and depend on the myeloid transcription factor PU.1. However, a recent study reported the existence of mrc1+ microglia in zebrafish embryos, which are seemingly independent of Pu.1 and reliant on lymphatic vessels, sparking great interest in the possibility of lymphatic-originated microglia. To address this, we took advantage of a pu.1 knock-in zebrafish allele for a detailed investigation. Our results conclusively showed that almost all zebrafish embryonic microglia (~95% on average) express pu.1. Further, lineage tracing and mutant analysis revealed that these microglia neither emerged from nor depended on lymphatic vessels. In essence, our study refutes the presence of pu.1-independent but lymphatic-dependent microglia.
Genes / Markers
Marker Marker Type Name
ccbe1GENEcollagen and calcium binding EGF domains 1
flt4GENEfms related receptor tyrosine kinase 4
spi1aGENESpi-1 proto-oncogene a
spi1bGENESpi-1 proto-oncogene b
1 - 4 of 4
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Figures
Figure Gallery (4 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cq25TgTransgenic Insertion
    hkz015tTgTransgenic Insertion
      hkz017tTgTransgenic Insertion
        hkz020tTgTransgenic Insertion
          hkz24
            		Small Deletion
            hkz25
              		Indel
              hkz032tTgTransgenic Insertion
                hkz036tTgTransgenic Insertion
                  ihb439
                    		Small Deletion
                    s896TgTransgenic Insertion
                      1 - 10 of 15
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      spi1bCRISPR10-spi1bCRISPR
                      1 - 1 of 1
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                      Fish
                      Fish
                      y1Tg/y1Tg
                      1 - 1 of 1
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                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab3-lcp1polyclonalRabbit
                      Ab-4C4monoclonal
                        		IgG1Mouse
                        1 - 2 of 2
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                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        CreEFGCre
                        DsRedEFGDsRed
                        DsRedxEFGDsRedx
                        EGFPEFGEGFP
                        GAL4EFGGAL4
                        GFPEFGGFP
                        mCherryEFGmCherry
                        1 - 7 of 7
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                        Mapping
                        No data available
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                        Citation
                        Yu, T., Chen, J., Wang, Y., Xu, J. (2024) The embryonic zebrafish brain is exclusively colonized by pu.1-dependent and lymphatic-independent population of microglia. Science advances. 10:eado0519.