PUBLICATION
The embryonic zebrafish brain is exclusively colonized by pu.1-dependent and lymphatic-independent population of microglia
- Authors
- Yu, T., Chen, J., Wang, Y., Xu, J.
- ID
- ZDB-PUB-240829-7
- Date
- 2024
- Source
- Science advances 10: eado0519 (Journal)
- Registered Authors
- Xu, Jin, YU, Tao
- Keywords
- none
- MeSH Terms
-
- Proto-Oncogene Proteins*/genetics
- Proto-Oncogene Proteins*/metabolism
- Microglia*/metabolism
- Lymphatic Vessels*/cytology
- Lymphatic Vessels*/embryology
- Lymphatic Vessels*/metabolism
- Embryo, Nonmammalian/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish*/embryology
- Trans-Activators*/genetics
- Trans-Activators*/metabolism
- Animals
- Brain*/embryology
- Brain*/metabolism
- PubMed
- 39196933 Full text @ Sci Adv
Citation
Yu, T., Chen, J., Wang, Y., Xu, J. (2024) The embryonic zebrafish brain is exclusively colonized by pu.1-dependent and lymphatic-independent population of microglia. Science advances. 10:eado0519.
Abstract
Microglia, the crucial immune cells inhabiting the central nervous system (CNS), perform a range of vital functions, encompassing immune defense and neuronal regulation. Microglia subsets with diverse functions and distinct developmental regulations have been identified recently. It is generally accepted that all microglia originate from hematopoiesis and depend on the myeloid transcription factor PU.1. However, a recent study reported the existence of mrc1+ microglia in zebrafish embryos, which are seemingly independent of Pu.1 and reliant on lymphatic vessels, sparking great interest in the possibility of lymphatic-originated microglia. To address this, we took advantage of a pu.1 knock-in zebrafish allele for a detailed investigation. Our results conclusively showed that almost all zebrafish embryonic microglia (~95% on average) express pu.1. Further, lineage tracing and mutant analysis revealed that these microglia neither emerged from nor depended on lymphatic vessels. In essence, our study refutes the presence of pu.1-independent but lymphatic-dependent microglia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping