PUBLICATION
Behavioral Assays Dissecting NMDA Receptor Function in Zebrafish
- Authors
- Zoodsma, J.D., Gomes, C.I., Sirotkin, H.I., Wollmuth, L.P.
- ID
- ZDB-PUB-240511-5
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2799: 243255243-255 (Chapter)
- Registered Authors
- Sirotkin, Howard
- Keywords
- Learning, MK-801, Prey capture, Social behavior, Social interaction, Zebrafish
- MeSH Terms
-
- Animals
- Brain/drug effects
- Brain/metabolism
- Zebrafish*/metabolism
- Receptors, N-Methyl-D-Aspartate*/metabolism
- High-Throughput Screening Assays/methods
- Behavior, Animal*/drug effects
- Larva/metabolism
- PubMed
- 38727911 Full text @ Meth. Mol. Biol.
Citation
Zoodsma, J.D., Gomes, C.I., Sirotkin, H.I., Wollmuth, L.P. (2024) Behavioral Assays Dissecting NMDA Receptor Function in Zebrafish. Methods in molecular biology (Clifton, N.J.). 2799:243255243-255.
Abstract
Zebrafish are a powerful system to study brain development and to dissect the activity of complex circuits. One advantage is that they display complex behaviors, including prey capture, learning, responses to photic and acoustic stimuli, and social interaction (Dreosti et al., Front Neural Circuits 9:39, 2015; Bruckner et al., PLoS Biol 20:e3001838, 2022; Zoodsma et al., Mol Autism 13:38, 2022) that can be probed to assess brain function. Many of these behaviors are easily assayed at early larval stages, offering a noninvasive and high-throughput readout of nervous system function. Additionally, larval zebrafish readily uptake small molecules dissolved in water making them ideal for behavioral-based drug screens. Together, larval zebrafish and their behavioral repertoire offer a means to rapidly dissect brain circuitry and can serve as a template for high-throughput small molecule screens.NMDA receptor subunits are highly conserved in zebrafish compared to mammals (Zoodsma et al., Mol Autism 13:38, 2022; Cox et al., Dev Dyn 234:756-766, 2005; Zoodsma et al., J Neurosci 40:3631-3645, 2020). High amino acid and domain structure homology between humans and zebrafish underlie conserved functional similarities. Here we describe a set of behavioral assays that are useful to study the NMDA receptor activity in brain function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping