PUBLICATION
Investigation of Physical Characteristics and In Vitro Anti-Inflammatory Effects of Fucoidan from Padina arborescens: A Comprehensive Assessment against Lipopolysaccharide-Induced Inflammation
- Authors
- Lee, H.G., Liyanage, N.M., Yang, F., Kim, Y.S., Lee, S.H., Ko, S.C., Yang, H.W., Jeon, Y.J.
- ID
- ZDB-PUB-240328-4
- Date
- 2024
- Source
- Marine drugs 22(3): (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Padina arborescens, anti-inflammatory activity, fucoidan, functional food, structural characteristics
- MeSH Terms
-
- Animals
- Inflammation
- Lipopolysaccharides*
- Nitric Oxide
- Phaeophyceae*
- Polysaccharides
- Zebrafish
- PubMed
- 38535450 Full text @ Mar. Drugs
Citation
Lee, H.G., Liyanage, N.M., Yang, F., Kim, Y.S., Lee, S.H., Ko, S.C., Yang, H.W., Jeon, Y.J. (2024) Investigation of Physical Characteristics and In Vitro Anti-Inflammatory Effects of Fucoidan from Padina arborescens: A Comprehensive Assessment against Lipopolysaccharide-Induced Inflammation. Marine drugs. 22(3):.
Abstract
A biocompatible, heterogeneous, fucose-rich, sulfated polysaccharide (fucoidan) is biosynthesized in brown seaweed. In this study, fucoidan was isolated from Padina arborescens (PAC) using celluclast-assisted extraction, purified, and evaluated for its anti-inflammatory potential in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Structural analyses were performed using Fourier transform infrared (FTIR) and scanning electron microscopy. Among the purified fucoidans, fucoidan fraction 5 (F5) exhibited strong inhibitory activity against LPS-induced nitric oxide (NO) production and pro-inflammatory cytokine generation through the regulation of iNOS/COX-2, MAPK, and NF-κB signaling in LPS-induced RAW 264.7 cells. Determination of the structural characteristics indicated that purified F5 exhibited characteristics similar to those of commercial fucoidan. In addition, further analyses suggested that F5 inhibits LPS-induced toxicity, cell death, and NO generation in zebrafish models. Taken together, these findings imply that P. arborescens fucoidans have exceptional anti-inflammatory action, both in vitro and in vivo, and that they may have prospective uses in the functional food sector.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping