PUBLICATION

Formation of different polyploids through disrupting meiotic crossover frequencies based on cntd1 knockout in zebrafish

Authors
Ou, Y., Li, H., Li, J., Dai, X., He, J., Wang, S., Liu, Q., Yang, C., Wang, J., Zhao, R., Yin, Z., Shu, Y., Liu, S.
ID
ZDB-PUB-240301-6
Date
2024
Source
Molecular Biology and Evolution   41(3): (Journal)
Registered Authors
Wang, Jing, Yin, Zhan
Keywords
cntd1, meiotic crossover, polyploidization, polyploidy, unreduced egg
MeSH Terms
  • Triploidy*
  • Animals
  • Seeds
  • Tetraploidy
  • Male
  • Ploidies
  • Zebrafish*
  • Polyploidy
  • Female
(all 9)
PubMed
38421617 Full text @ Mol Bio Evol
Abstract
Polyploidy, a significant catalyst for speciation and evolutionary processes in both plant and animal kingdoms, has been recognized for a long time. However, the exact molecular mechanism that leads to polyploid formation, especially in vertebrates, is not fully understood. Our study aimed to elucidate this phenomenon using the zebrafish model. We successfully achieved an effective knockout of the cyclin N-terminal domain containing 1 (cntd1) using CRISPR/Cas9 technology. This resulted in impaired formation of meiotic crossovers, leading to cell-cycle arrest during meiotic metaphase and triggering apoptosis of spermatocytes in the testes. Despite these defects, the mutant (cntd1-/-) males were still able to produce a limited amount of sperm with normal ploidy and function. Interestingly, in the mutant females, it was the ploidy, not the capacity of egg production that was altered. This resulted in the production of haploid, aneuploid, and unreduced gametes. This alteration enabled us to successfully obtain triploid and tetraploid zebrafish from cntd1-/- and cntd1-/-/- females, respectively. Furthermore, the tetraploid-heterozygous zebrafish produced reduced-diploid gametes and yielded all-triploid or all-tetraploid offspring when crossed with wild-type (WT) or tetraploid zebrafish, respectively. Collectively, our findings provide direct evidence supporting the crucial role of meiotic crossover defects in the process of polyploidization. This is particularly evident in the generation of unreduced eggs in fish and, potentially, other vertebrate species.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
cntd1CRISPR1-cntd1CRISPR
1 - 1 of 1
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Fish
No data available
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab2-sycp3polyclonal
    		IgGRabbit
    Ab3-mlh1monoclonal
      		IgG2aMouse
      1 - 2 of 2
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      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
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      Citation
      Ou, Y., Li, H., Li, J., Dai, X., He, J., Wang, S., Liu, Q., Yang, C., Wang, J., Zhao, R., Yin, Z., Shu, Y., Liu, S. (2024) Formation of different polyploids through disrupting meiotic crossover frequencies based on cntd1 knockout in zebrafish. Molecular Biology and Evolution. 41(3):.