PUBLICATION

Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons

Authors
Bin, J.M., Suminaite, D., Benito-Kwiecinski, S.K., Kegel, L., Rubio-Brotons, M., Early, J.J., Soong, D., Livesey, M.R., Poole, R.J., Lyons, D.A.
ID
ZDB-PUB-240228-7
Date
2024
Source
Nature communications   15: 17901790 (Journal)
Registered Authors
Kegel, Linde, Lyons, David A., Poole, Richard
Keywords
none
MeSH Terms
  • Zebrafish*
  • Neurons
  • Axons*/physiology
  • Animals
  • Myelin Sheath/physiology
  • Central Nervous System
(all 6)
PubMed
38413580 Full text @ Nat. Commun.
Abstract
Axon diameter influences the conduction properties of myelinated axons, both directly, and indirectly through effects on myelin. However, we have limited understanding of mechanisms controlling axon diameter growth in the central nervous system, preventing systematic dissection of how manipulating diameter affects myelination and conduction along individual axons. Here we establish zebrafish to study axon diameter. We find that importin 13b is required for axon diameter growth, but does not affect cell body size or axon length. Using neuron-specific ipo13b mutants, we assess how reduced axon diameter affects myelination and conduction, and find no changes to myelin thickness, precision of action potential propagation, or ability to sustain high frequency firing. However, increases in conduction speed that occur along single myelinated axons with development are tightly linked to their growth in diameter. This suggests that axon diameter growth is a major driver of increases in conduction speeds along myelinated axons over time.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nkhspGFF62ATgTransgenic Insertion
    nkuasgfp1aTgTransgenic Insertion
      nkuasrfp1aTgTransgenic Insertion
        ue2TgTransgenic Insertion
          ue57
            		Point Mutation
            ue76
              		Small Deletion
              ue77
                		Small Deletion
                w2
                  		Point Mutation
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  ipo13bCRISPR1-ipo13bCRISPR
                  ipo13bCRISPR2-ipo13bCRISPR
                  1 - 2 of 2
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                  Fish
                  No data available
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  EGFPEFGEGFP
                  GAL4FFEFGGAL4FF
                  RFPEFGRFP
                  1 - 3 of 3
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                  Mapping
                  No data available
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                  Citation
                  Bin, J.M., Suminaite, D., Benito-Kwiecinski, S.K., Kegel, L., Rubio-Brotons, M., Early, J.J., Soong, D., Livesey, M.R., Poole, R.J., Lyons, D.A. (2024) Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons. Nature communications. 15:17901790.